Part:BBa_K3978013:Design

BL21 vector combined with amoA and hao gene sequence to degrade EE2 in wastewater

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 112
  Illegal XbaI site found at 30
  Illegal PstI site found at 131
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 112
  Illegal PstI site found at 131
  Illegal NotI site found at 149
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 112
  Illegal BglII site found at 305
  Illegal BamHI site found at 106
  Illegal XhoI site found at 354
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 112
  Illegal XbaI site found at 30
  Illegal PstI site found at 131
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 112
  Illegal XbaI site found at 30
  Illegal PstI site found at 131
  Illegal NgoMIV site found at 324
  Illegal NgoMIV site found at 671
  Illegal NgoMIV site found at 5348
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1256
  Illegal SapI.rc site found at 2916
  

Design Notes

use electrotransformation can effectively transform the sequence into BL21

Source

the pETDuet vector T7 promoter is embeded with amoA and hao gene sequence which is submitted to webside and named BBa_K3978011 and BBa_K3978011